SAP130 Antikörper (AA 309-913)

Produktnummer ABIN7601247

Dieser Kaninchen Polyklonal Antikörper erkennt spezifisch SAP130 in WB, ELISA, IHC, IF, ICC und FACS. Er zeigt eine Reaktivität gegenüber Human, Maus und Ratte.

Kurzübersicht für SAP130 Antikörper (AA 309-913) (ABIN7601247)

Target: SAP130 (Sin3A-Associated Protein, 130kDa (SAP130))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SAP130 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Produktdetails anti-SAP130 Antikörper

Bindungsspezifität: AA 309-913

Verwendungszweck: Anti-SAP130 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-S Antibody Picoband® (ABIN7601247). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human SAP130 recombinant protein (Position: H309-H913).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.25 μg/mL, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Fleischer, T. C., Yun, U. J., Ayer, D. E. Identification and characterization of three new components of the mSin3A corepressor complex. Molec. Cell Biol. 23: 3456-3467, 2003. 2. Metselaar, P. I., Hos, C., Welting, O., Bosch, J. A., Kraneveld, A. D., de Jonge, W. J., Te Velde, A. A. Ambiguity about splicing factor 3b subunit 3 (SF3B3) and Sin3A associated protein 130 (SAP30). Cells 10: 590, 2021. 3. Seifert, L., Werba, G., Tiwari, S., Giao Ly, N. N., Alothman, S., Alqunaibit, D., Avanzi, A., Barilla, R., Daley, D., Greco, S. H., Torres-Hernandez, A., Pergamo, M., and 10 others. The necrosome promotes pancreatic oncogenesis via CXCL1 and Mincle-induced immune suppression. Nature 532: 245-249, 2016. Note: Erratum: Nature 591: E28, 2021.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Details zu SAP130

Target: SAP130 (Sin3A-Associated Protein, 130kDa (SAP130))

Andere Bezeichnung: SAP130

Hintergrund:

Synonyms: C-C motif chemokine 16, Chemokine CC-4, HCC-4, Chemokine LEC, IL-10-inducible chemokine, LCC-1, Liver-expressed chemokine, Lymphocyte and monocyte chemoattractant, LMC, Monotactin-1, MTN-1, NCC-4, Small-inducible cytokine A16, CCL16, ILINCK, NCC4, SCYA16

Tissue Specificity: Mainly expressed in liver, also found in spleen and thymus. Highly expressed in LPS- and IFN-gamma- activated monocytes, weakly in some lymphocytes, including natural killer cells, gamma-delta T-cells, and some T-cell clones.

Background: Histone deacetylase complex subunit SAP130 is an enzyme that in humans is encoded by the SAP130 gene. This gene encodes subunit 3 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. Subunit 3 has also been identified as a component of the STAGA (SPT3-TAF(II)31-GCN5L acetylase) transcription coactivator-HAT (histone acetyltransferase) complex, and the TFTC (TATA-binding-protein-free TAF(II)-containing complex). These complexes may function in chromatin modification, transcription, splicing, and DNA repair.

Molekulargewicht: 130 kDa

Gen-ID: 79595

UniProt: Q9H0E3